Please do not split your case with multiple topics/treads.
Use your other one after we deal with this narrow question.
The whole objective is to get steadier levels and avoiding peaks.
Peaks make E2 problems worse and can make hematocrit worse for those who have such problems.
Peaks really do nothing good.
Peaks of T are followed by peaks in E2 and as T levels drop in a cesspool of estrogens you feel like shit. At least that is how it plays out most of the time.
When using anastrozole to manage E2 levels to get near E2=22pg/ml, which works well for almost all guys, the competitive drug needs to match FT levels. If FT, or Bio-T is not steady, anastrozole dose is not well matched and then E2 is not managed well. For most guys, E2 management is mission critical.
When hormone levels change during the week, your lab results are mostly a function of lab timing, making them rather useless. And to reduce that effect, one should always do labs 1/2 way between injections to reduce lab timing artifacts.
When some are injecting 250iu hCG SC/SQ EOD, they then do T and anastrozole on an EOD basis to keep routine simplified. But twice a week is fine is that is what you want to do.