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Supplement Increases Testicular Size


Effects of nutritional supplements on testicular size and the secretion of LH and testosterone in Merino and Booroola rams

Graeme B. Martin1, Stephen R.D. Sutherland2, David R. Lindsay2Accepted 17 June 1986.

Six Booroola and six Merino rams were fed either a diet which maintained constant live weight or the same diet plus a supplement of high protein lupin grain for 15 weeks, and changes in live weight and testicular volume were measured. Serial blood samples taken for 24 h before the start and 9 weeks after the treatment began were assayed for plasma LH and testosterone and the resulting profiles were analysed for pulses of both hormones. Five weeks later, the animals were given two intravenous injections of 1 μg gonadotrophin-releasing hormone (GnRH) 1 h apart in order to measure pituitary gland responsiveness. A further week later the animals were injected intravenously with 500 μg human chorionic gonadotrophin (hCG) and the levels of testosterone were measured in samples taken after 1.5 h to estimate the testicular responsiveness.
The nutritional supplement stimulated testicular growth in both genotypes, so that at the end of the treatment period the testes had increased significantly (P<0.01) in volume by 66% in the Merinos and by 63% in the Booroolas. The live weights also increased, but by relatively less (34% and 43% for supplemented Merinos and Booroolas). The rates of increase in both testicular size and live weight were similar for the two breeds. There were no significant effects of diet on the tonic secretion of LH or testosterone, or on responsiveness to GnRH or hCG.
The intervals between LH pulses were significantly shorter (P<0.05) in Booroola rams than in Merino rams both before and after treatment (5.8 h vs. 11.6 h before treatment). The breed differences in LH secretion were mimicked by the testosterone profiles. In the Booroolas, five of the twelve LH profiles contained groups consisting of two to four individually identifiable pulses, each of which elicited a separate pulse of testosterone. A pulse group was observed in only one profile from the Merinos (P=0.06). There were no significant differences between the genotypes in any other parameter of LH or testosterone secretion, or in their responsiveness to GnRH or hCG.
It was concluded that (i) nutritional supplements will stimulate testicular growth in both Merino rams and Booroola rams; (ii) the increase in testicular size does not appear to involve an increase in the responsiveness of the testis to LH; and (iii) there are both qualitative and quantitative differences between the genotypes in the patterns of secretion of LH and testosterone which may be associated with the differences in their fecundity.

similar excerpt:
In 6 Australian Merino rams given a roughage diet with 750 g lupin (Lupinus angustifolius) daily for 5 weeks liveweight increased by 29% and testicular size by 58%, and the mean number of lutropin pulses in 24 h increased from 1.3 to 2.7. The control Merino rams showed no changes. In the Booroola rams given lupin liveweight increased by 23% and testicular size by 63%. In the control Booroola rams liveweight did not change but testicular size increased by 32% and the difference in testicular size between supplemented and control Booroola rams after 9 weeks was not significant. The mean frequency of lutropin pulses in Booroola rams was initially higher than in Merino rams but did not change with supplementation
It is actually used very frequently and devisively by farmers:
Lupin supplementation is often used to increase ram testes volume and
fertilizing ability. A supplement fed at the rate of 500 g lupin grain per day for 8-10 weeks can
increase testes volume in Merinos from around 400 ml to about 600 ml. This should enable
the use of fewer rams at joining, and the saving related to purchasing fewer rams easily
covers the cost of feeding the supplement.
why not the same for us?

HAS THIS EVER BEEN TRIED ON HUMANS? and why on earth not? a simple and natural, cheap nutritional supplement.

500g lupin protien every day for 5 weeks and double the size of your balls

please help me research this


Thank you for this.


Hahaha, yes it is true - we do use lupin here to increase testicular volume in animals.
Some of my colleagues are the key researchers trying to get lupin mainstream for human consumption.


500g would be quite a bit you know?

Is the premise that the bigger the balls the more test you'll have?


I believe the frequent use revolves around fertility. Whether sperm count or quality increases I am not sure. But testicular volume is a quality that breeders judge. They can gain or lose a lot of money on things like this.


I'm sorry, are you guys thinking that because this was seen in rams that humans need to start taking it?

What is the point of this thread? You can find tons of studies about just about anything. It doesn't mean humans should use the info as having direct correlation and effect across species like this.


To be fair, he isn't saying it WILL work, he's just posing the question.


I understand exactly what he is doing and his comments along with the comments of a few others imply a fundamental misstep in the comprehension of how scientific studies even work.

This is an animal study. Regardless of what the outcome is, you don't take a biologic animal study like this and immediately apply it to humans.

"Posing the question" only works if we have human case studies. Otherwise, there is no question to pose.


That doesn't follow. If you already have human case studies, the question must have already been posed.



His question is irrelevant unless you or someone here is about to conduct a study on humans. Get it?

Some of us actually went to school for this. I'm just trying to help YOU out.


He's saying that Lupin has shown to increase ram testicle size, so why not in humans? This seems like a perfectly reasonable question to me. There's nothing irrelevant about asking simply because he hasn't found someone already willing to do such a study (in fact he's asking for help studying this).

Again, he's not saying it WILL work for us, he's asking why not for us and if such a study has already been done.


lol so any scientific inquiry in irrelevant unless there is a human trial imminent...right. There's no question to pose? How about the question, gee I wonder if this can work in humans? Seems like a pretty reasonable question to me

Guys, no more questions unless you've previously emailed every research lab in the world to ensure that a human trial is underway already. Let the people who went to school for this stuff handle it...rookies


Is the point this hard to understand? Are there studies on humans? That is the only relevant question here, not asking random people on the internet if it is possible. Anything is possible. Everything isn't probable.

This can not be this difficult.

I can go find a study about how caffeine makes the penises of elk grow 2" and asking random people on the net if it is possible for it to apply to humans is still as pointless as this thread unless there are some human studies to discuss.


Also, if I am making the wrong assumption that some believe this translates directly to humans, I apologize, because that is what I am arguing against, not the simple discussion of a study.