thank you for the answers so far…
what do you mean by HPLC?
My idea on this creatinine level measurement was this:
Unused Creatine or creatine out of the muscel cells which has been used converts into creatinine and shows up in urine measurements.
So you could derive from the creatinine levels in your urine how much of the supplemented creatine was actually used (stored in muscle)… right?
I know there are many factors, like kidney function and physical activity.
But I only want to compare the results in relation to each other…
Like: using creatine with high carb gets about 2x less creatine levels, so a greater amount of creatine must have been used in muscle…
what do you think of this?
Are there any other possibilities for doing something scientifical with creatine coming into your mind? I’m also thinking of a survey… but this should be the last option…
thank you very much…[/quote]
forget i mentioned HPLC it would be too difficult and troublesome to get your hands on that type of apparatus, “High pressure Liquid Chromatography” it’s just a purification method, it seperates out individual compounds if you have a mixture.
Obviously to measure the effect of supplemented creatine you would require a control, due to the body making it’s own creatine would show up in urine as well.
This is where it would be difficult somewhat, unless you did all the different measurements on the same subjects instead of dividing people into groups. I have read that creatine synthesis is markedly different between individuals. So in effect you would have to administer placebo, measure urinary creatinine with that individual, probably in g/ml if the numbers are logical to do so. Then you administer supplemental creatine with high carbs for instance, and then measure urinary creatine.
This is another thing you have to think about: lets say backround creatinine concentration in the urine is 5 micrograms/ml. You supplement creatine and suddenly you see it jumps to 50micrograms/ml creatinine. This does not neccessarily mean all 45 micrograms/ml increase was due to muscle tissue substrate phosphorylation.
A question to think about here. Is when creatine is cyclically condensed to creatinine in the stomach, can creatinine be absorbed into the blood as well as creatine? or is creatinine excreted and only creatine absorbed.
Something tells me some creatinine is absorbed and will effect your urinary results. You cannot postulate that the total increase in urinary creatinine was due to active phosphorylation.
I’m really interested in this study, maybe it could be simplified somewhat, and i will think about how. feel free to PM me at any time if you feel like i could help.
apparently they do make creatinine test strips, so hopefully the range they test for will coincide with your tests.
I would like to test the following on 1 individual:
making sure to keep diet completely consistent:
backround creatinine concentration(placebo)
backround creatinine concentration(placebo)+ weight training
supplemented creatine and resulting creatinine concentration
supplemented creatine and resulting creatinine concentration after weight training.
This would allow you to figure out how much was used during weight training, as i’m sure after a couple hours of supplementation, creatinine produced through metabolic condensation would be gone.