I see two issues regarding the shelf life of dry hCG reconstituted with preservative free normal saline: sterility and potency.
First, sterility. There is no preservative in the NS. With careful sterile technique and strict refrigeration at 33-38F, I am hoping to get a month out of it. I am coming up on my first month now and there has been no signs of skin irritation or redness that might indicate bacterial growth. So far, so good.
Next, potency. I assume that the hCG polypeptide chain will undergo slow hydrolysis at refrigerator temperature and lose potency over time, but how much? I have no idea. I have thought to increase the dose 10% per ten days, or something like that, but really I would be just shooting from the hip. What have others done? Anyone have any knowledge about the shelf life of peptide chains floating in NS?