T Nation

Prolotherapy Supplementation

I am currently getting prolotherapy treatments for back and shoulder pain and was wondering if there were any supplementation that I could utilize to help the process. I am hoping to do 2 things- boost immune response and boost collagen production. I have read some articles about certain AAS increasing collagen production, but others can actually decrease collagen production. I do not have access to AAS, and do not wish to partake in them solely for legal reasons, but was hoping that someone could help me through some chemistry with certain supplements.

Does anyone have any idea if TRIBEX, Alpha Male, REZ-V, BCAA or even MAG-10 have any positive influence on collagen production? Also, does anyone have some suggestions for immune boosting supplements? I have access to glutathione injections and plan on partaking in them, but other suggestions would be greatly appreciated.

To boil it down to bullet points:

  1. Does anyone have input on supplements that increase collagen production (androgenic/anabolic or otherwise)?
  2. Does anyone have input on supplements that bolster immune response?
  3. Any supplements to avoid that could detrimentally influence collagen production?
    As always, all thoughts are greatly appreciated.

a very solid hypercaloric diet.

gluco… Chondroitin. MSM mix.

knaw on bones and bone marrow :slight_smile:

Fish oil supplementation

those will be huge

mesenchymal stem cell therapy, Shark cartilage suppliments, But nothing beats good old vit c, fish oil, and reducing the over all inflamation in the body…

Prolotherapy…interesting stuff…it’s not really known for sure if its effects have to do entirely with promoting collagen synthesis and subsequent tissue stiffening/stabilization or if it simply obliterates pain receptors within the target tissues…

Anyway, while not specifically linked with prolotherapy, here’s an article reference and abstract that might interest you:

Ann Surg. 2002 Sep;236(3):369-74; discussion 374-5.
Effect of a specialized amino acid mixture on human collagen deposition.
Williams JZ, Abumrad N, Barbul A.
Department of Surgery, Sinai Hospital of Baltimore, Johns Hopkins Medical Institutions, Baltimore, Maryland 21215, USA.

OBJECTIVE: To examine the effect of arginine, beta-hydroxy-beta-methylbutyrate (HMB), and glutamine supplementation on wound collagen accumulation in a double-blind, randomized study. SUMMARY BACKGROUND DATA: Control of wound collagen synthesis has been an elusive goal for clinicians and scientists alike. In many clinical instances, it is desired to increase collagen deposition as a means of enhancing wound strength and integrity. Arginine, a semiessential amino acid, has been shown to increase wound collagen accumulation in rodents and humans. HMB, a metabolite of leucine, regulates muscle proteolysis in animals and humans and increases collagen deposition in rodents. METHODS: Thirty-five healthy, nonsmoking human volunteers 70 years or older were enrolled and underwent subcutaneous implantation of two small, sterile polytetrafluoroethylene (PTFE) tubes into the deltoid region under strict aseptic techniques. The tubes were 1 mm in diameter and 6 cm in length with pore size of 90 to 120 microm to allow optimal ingrowth of fibroblasts and the deposition of matrix. Eighteen volunteers (mean age 75.4 years; 2 men, 16 women) were randomized to receive daily supplementation of 14 g arginine, 3 g HMB, and 14 g glutamine (total nitrogen 3.59 g) in two divided doses. The control group (n = 17; mean age 75.3 years; 6 men, 11 women) received an isonitrogenous, isocaloric supplementation of nonessential amino acids. Catheters were removed at 7 and 14 days postimplantation and analyzed for hydroxyproline (OHP, nmol/cm catheter, an index of collagen accumulation) and alpha-amino nitrogen (alpha-AN, mmol/cm, an index of total protein deposition). RESULTS: Supplements were well tolerated, without any reported side effects. Supplementation with the specialized amino acid mixture led to a significant rise in plasma arginine and ornithine levels. The specialized amino acid supplement led to a significant increase in collagen deposition (as reflected by OHP content) in the PTFE tubes without an effect on total protein accumulation. CONCLUSIONS: Collagen synthesis is significantly enhanced in healthy elderly volunteers by the oral administration of a mixture of arginine, HMB, and glutamine. This provides a safe nutritional means for increasing wound repair in patients.

Hey guys, sorry for the late reply…
I am on a solid hypercaloric diet and cramming as much as fish oil down my gullet as I can, so I think I have those bases covered. I am gonna buy me a chicken and start gnawin on some bones, too :slight_smile:

I am going to research the glutamine mix some- I am always weary of drawing results across different populations…
Has anybody read anything or have any input on hydrolyzed collagen? Or any input on cAMP impact on collagen production? I managed to locate 2 studies on elevated cAMP activity and collagen production.

One indicated it adversely impacted and the other one indicated it positively impacted… Unfortunately I could only get partial reports because I am not a member of the dern magazine and couldnt find it on pubmed…
Anyhoo- thanks for your thoughts and have a good one!

I just finished a book on prolotherapy called Prolo Your Pain Away, by Ross A. Hauser and Marion A. Hauser. In it they suggest one or more of the following supplements to use before and after having prolotherapy: Bromelain, Trypsin, Papain, and a supplement called Prolo-max that you can find at benuts.com. I haven’t had the time to do any research on any of their suggestions. Hope this helps.

Painless- I actually get treatments from a guy who was trained w/ the author of that book. the prolo is helping, most definitely, and I will start reading up on those supps. Thanks.