I don't think so. You may have shortened the life of it, though, but I doubt even this. THe only other thing I could think of is the HCG powder might not go fully into solution.
Keep in the fridge and in the dark.
I always get a pleasant ache and fullness the day after HCG. If that doesn't happen, chunk it.
(FWIW, I think mine always comes with just sterile water; I will look when I get home.)