You should be injecting 50mg twice weekly, whenever you inject once weekly you T and E2 will peak within 48 hours and then it will start falling and towards the end of the week you could start feeling low because your levels are lower. The only instance where once weekly is ideal is someone who’s SHBG level is at or above the top of the range since they tend to hold on to their T well. That’s a perfect SHBG level for anyone going on TRT!