250iu hCG SC EOD was shown to be a LH replacement dose. So that natural level of LH receptor activation is thought not be a problem.
If one wants to take less hCG, the benefit would be less cost and for those who get too much testicular E2 generation with hCG, a lower dose might solve that issue. [This is where I have been suggesting that lower doses should be explored.]
Another approach would be a lower dose that still keeps the testes in an acceptable physical state.
Pregnenolone levels depend on many other things, such as CpQ10 levels, anti-oxidant levels and many other things. So that is not going to be a dose driver.