This wasn’t done correctly, levels peak in 24-48 hours, levels should be measured at trough.
This is the wrong approach, don’t try to get your levels in range, increase your dosage up or down until symptoms are gone, then run the numbers to see what feeling optimal looks like on paper.
When I was dialing in my daily protocol, I didn’t check my levels when not feeling well, I increased/decreased the dosage until I felt good, then I ran my labs and found the 400-500 range to be best.
If you get to a point where sides are unmanageable, you probably need more frequent smaller injections.