NOTE: DO NOT EVEN CONSIDER USING DNP IF YOU’RE UNDER 25 YEARS OF AGE. IF YOU ARE YOUNGER, HOT-ROX EXTREME IS FOR YOU AND WORKS WELL FOR MOST OF US. DNP HAS ONE MAIN SIDE EFFECT- DEATH DUE TO BODY OVER-HEATING. READ EVERYTHING ON THIS PAGE IF YOU’RE STILL CONSIDERING USING DNP. YOU HAVE BEEN ADVISED…
Another note. I feel obliged to tell ya this- Treat the following article as some ‘food for thought’. If you want to confirm if the below is true, use Pubmed for actual published articles from valid sources. Yes, the following information may be flawed and not 100% correct. What you going to read isn’t in anyway a ‘DNP bible’, far from it…its to give an idea to prospective users of what they should research WHEN USING SAFE AND TRUSTED SOURCES OF INFORMATION (Pubmed for example). Take the following with a pinch of salt. Upon further thought, this article doesn’t deserve to be stickied. No sense. Good stickied articles have lots of actual users posting in the same thread allowing for dynamic change of the thread based upon personal experience AND actual published articles FROM TRUSTED SOURCES. The following has no guarantee to be written by a trusted source, and yes, it may have wrong info too. Treat the article as some ‘food for thought’.
Cutting down made extremely effective-
and extremely dangerous: A couple of
words from Bicep_craze.
Well, I had been doing some research on this stuff and came over this info. I am not taking any credit for the following information.
Why am I doing this?
I hope it will help those that are considering running a cycle with this deadly, yet extremely effective compound. If you’re extreme enough to use DNP, you might as well be informed. Again DNP is one of those things that work, and work well, but may easily cook you from the inside out if not used properly.
Also it is a highly flammable substance, so it MUST BE HANDLED WITH CARE. Feel free to add info and personal experiences with this stuff in this thread, if you have any.
The following link has a very informative log of a guy that used DNP. The guy lost 6Kgs in just 11 days with no exercise, estimated bf %: This guy says he went down from 20% to 15% body fat, or at least he states so. Again, this is a deadly compound since its effective dose is just slightly less than the lowest reported deadly dose.
READ THAT AGAIN:
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
This is a deadly compound since its effective dose is just slightly less than the lowest reported deadly dose.
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Here’s the link for the log of this guy:
musclechat.co.uk/performance-enhancement-anabolic
-steroids/20118-super-fat-loss-my-dnp-log-4.html
Also Anthony Roberts had written an article about his personal experience with DNP a while back. Here’s the link:
Anthony Robert’s himself has defined using this compound witought any knowladge as “Committing suicide with 1 easy step” in one forum post on T-Nation where a guy apparently gulped down 500mg of DNP at once on it’s first day when he/she should have been assessing personal tolerance to DNP. The latter was the thread OP by the way and stated that 20 minutes after gulping down the capsule he was feeling the heat. No this isn’t bullshit. Ah, did I forget to tell that he never posted anything again?
Stay strong, and read the following well. If you can’t bear reading EVERY SINGLE WORD of the following and the above linked articles, and then some, than forget about DNP. It isn’t for you.
Stay strong, and remember that knowledge is power.
Bicep_Craze
DNP
By
Monte E. DNP Guru
This is an educational article covering different aspects of DNP and is intended only to educate the reader about DNP. This article is far from comprehensive, but it should provide a good background to get the reader started on learning about DNP.
In this article I will attempt to cover the following topics regarding DNP:
History
Mechanism of Action
Dose and Cycle Recommendations
Dietary Recommendations
Side effects/ risks
Prevention/ Contraindications
Recommended supplements with DNP
HISTORY:
DNP stands for 2,4-dinitrophenol. This is a chemical that was once used in the early 20th century to ignite dynamite and cast a yellow dye on wood and other handcrafts. A few years later demographical statistics showed that employees who worked with DNP everyday tended to lose weight, often rapidly.
One fall out from this was a study conducted by Stanford University in 1920 showing that the ingestion of DNP does in fact cause weight loss. This prompted physicians to prescribe DNP to obese patients of that era.
DNP was on the market for 2 decades as a weight loss drug and was eventually taken off the market and banned for human consumption by the FDA because there was a report of cataract formation among female users of this drug which turned out to be false. This chemical is still deemed too dangerous by the FDA to allow it to come back to the pharmaceutical marketplace.
Over the decades of research on DNP, scientists have never shown it to have the ability to cause cancer or any other mutations despite the fact that its a phenol and that most phenolic compounds are carcinogenic.
DNP is now only used as a research chemical and as a pesticide in a few states that still approve of its use. It is not illegal to own DNP, but it is illegal to market it for personal consumption.
MECHANISM OF ACTION:
DNP exerts its effects within the cell, more specifically within the membrane of the mitochondria. The advantage of intracellular mechanisms of action such as this is that a tolerance to DNP cannot develop. To make a long story short, DNP makes the process of ATP formation very inefficient.
Why is this important? Because ATP is the energy unit needed to drive all our biochemical reactions in our body that is necessary to keep us alive. The cells in our body constantly need energy (ATP) to stay alive. The amount of ATP needed to keep a person alive depends on his/her basal metabolic rate.
By making ATP formation inefficient, a persons basal metabolic rate can increase indefinitely, but for practical uses, basal metabolic rate can safely increase by 30-50% without putting ones life in danger. It is not unheard of for people to lose up to one pound of pure fat per day while on DNP.
If youre not familiar with ATP, its what the Calories that are stored in carbs, fats, and proteins are eventually turned into. In other words, the energy that is stored in the macromolecules are transferred to the ATP molecule, but DNP disrupts this process.
Instead of making ATP from macromolecules in the presence of DNP, the potential energy is just turned into heat. This is very significant because ATP levels in the body will quickly diminish and cells want to replenish that storage by breaking down more fats, carbs, etc.
As you can see, a patter quickly develops where ATP levels will constantly be below normal and the body will always be trying to burn more fats, carbs, and proteins to help replenish the ATP levels. This is no different than doing aerobic exercises such as jogging, biking, etc, except while on DNP, the body is doing the aerobic exercise non stop 24 hours a day.
DOSES AND CYCLE RECOMMENDATIONS:
DNP is not a drug for everyone, definitely not the beginner who just wants to lose a couple of pounds to look better with the shirt off Without proper education on its use, DNP can be deadly.
There are 2 forms of DNP currently on the market, pure crystalline (100% dry) DNP, and powdered DNP (usually 5-10% moisture). The crystalline version is stronger and more effective, but more caution needs to be used while using it. It acts much faster, and the side effects also subside faster as well.
I recommend between 2-6mg/kg-bw per day for crystalline DNP and 4-10mg/kg-bw for powdered DNP. A beginner should always start off at the low end to assess tolerance. Trying this for the first time 2 weeks before a competition can be disastrous.
A 220lb man is 100kg exactly. This means that if he is a first time user of crystalline DNP then he should take 200mg per day. I suggest staying with this dose for at least 3 days to keep it safe, then slowly increase the dosage.
400mg/day can be used, but never take it all at once. Always split up the doses as far as possible, so for 400mg/day that would mean taking 1 200mg capsule every 12 hours.
Only on rare occasions should someone attempt 600mg/day with the crystalline capsules unless its used by a very experienced user and all the vital signs are closely monitored.
Cycle length depends largely on the individual. At first it was thought that a DNP cycle should be limited to 10 days at the most because the thyroids shuts down and t4 to t3 conversion in the liver becomes nil, however, this is not the case.
10 days is a very arbitrary number. A person taking 200mg/day would have almost completely normal thyroid function at day 10 whereas if s/he took 600mg/day, t3 would be non existent after 3 days.
While the t3 hormone plays a very large role in determining fat loss, it should not be a big concern while on DNP because the fat burning capabilities of DNP will more than compensate for the suppressed t3 levels.
An advantage to suppressed t3 levels is that the body will burn much less muscle while still burning fat on DNP. Normal t3 and thyroid function is restored within a week of stopping DNP.
Ok, so how long should you do it? I suggest playing around with it and just go by how your body feels. It is not a bad idea to just take 2-3mg/kg-bw for 3-4 weeks. This causes less side effects and will have the same overall effect, but it will just take a bit longer.
After you get used to 2-3mg/kg-bw, then another option is to up the dose by 1 cap and carrying that out for as long as your body can handle it because fatigue and a host of other side effects will eventually overtake you. If 2 caps/day is still too mild then repeat the above step with 3 caps per day spread out into 8-hour intervals.
Because of some water retention caused by DNP, users typically find that they look their best 4-7 days after finishing their cycle when the water has normalized.
For competitors:
Take the last DNP capsule 8 days prior to the competition date. Carb deplete after 3 days after the last cap. Carb load immediately 2 days prior to competition and stop fluid intake. This should allow for excellent glycogen super compensation within the muscles for a fuller look.
DIETARY RECOMMENDATIONS:
-
Carb deplete for 3 days prior to DNP because DNP will take a good 2-3 days to deplete the body’s glycogen stores before it can efficiently burn stored fat.
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Once on DNP eat an isocaloric diet (33% prot, 33% fat, 33% carbs) and keep the calories at around maintenance level. Restricting carbs will put the body in a state of hypoglycemia and can be dangerous to the health and also the mental well being. DNP also mimics insulin in that it shuttles glucose into the cells in the absence of glucose.
This is great for fat burning, but when carb intake is too low the blood glucose can be at dangerously low levels as well. a more experienced user can switch up this ratio a bit. Either way it won’t make a huge difference because it’s mostly about the total calorie consumption.
This is what Im proposing to be the optimal DNP diet (for a high dose short cycle(s) and the end of a low dose extended cycle only):
50% carbs, 35% protein, 15% fat. Its not a misprint; carbs are essential for DNP to work properly. Keep in mind that its only the percentage that changes and not the total calories.
From this point it will get a bit complicated, but read over it a few times and you will get the gist of it. Ill also try to keep it as simple as possible.
When fatty acids are broken down they need to be fed into an energy cycle for a complete break down so that more can be broken down later. The beginning of this cycle is called the citric acid cycle. Fats enter the citric acid cycle as a 2-carbon molecule called acetate and to start off this cycle it needs to bind to another 2-carbon molecule called oxaloacetate.
Without enough oxaloacetate this cycle cannot proceed. With little oxaloacetate this cycle is slowed down, thus fat burning is slowed down. Where does oxaloacetate come from? Several sources, but the main one is from pyruvate, the end product of the first step of glucose (carbohydrate) metabolism. Without enough glucose in the blood, fat burning becomes very inefficient.
This is not to say the more carbs we eat the more pyruvate we can generate, therefore the more fat is burned.
We only need adequate levels of pyruvate to supply the citric acid cycle of the necessary starting material for fat to enter, and then it will eventually proceed to be completely oxidized in the electron transport chain.
Dont worry about eating too many carbs while on DNP because these carbs cannot be stored and are immediately used for fuel to try to replenish cellular ATP. While keeping the calorie level at maintenance level, it would be most beneficial to eat about 55% calories from carbs, 35% protein and 10% fat (mostly unsaturated).
It may be optimal for fat burning to raise the carbs a bit more, but the protein should be high enough so that muscle catabolism is kept at a minimum when DNP creates the huge calorie deficit in the body.
The least effective form of dieting while on a DNP cycle is a fat diet, or ketogenic diet, but the high amounts of fat helps to slow gastric emptying, so you feel more satisfied for a longer period of time. This is one reason why I first recommended the isocaloric diet to beginners who may have trouble controlling their appetite while on DNP.
SIDE EFFECTS:
Heat- you will feel very hot while taking this. It is very similar to jogging a slow pace all day long, so be prepared to sweat a little. In some people a lot of sweat is not too uncommon. Body temperature will rise to about 101 degrees and sustain there.
This is not too out of the ordinary. This increase in core body temperature causes a vasodilation effect throughout the body to help cool you off. However, evaporative cooling with the aid of vasodilation will not be effective when the surrounding environment does not allow for proper cooling.
For example, being out in the summer sun when its 90 degrees and high humidity can cause you to rapidly overheat to dangerous levels. Avoid hot environments at all costs.
Stay indoors if you choose to use it in the summer and only go outside briefly when its absolutely necessary. Dehydration can cause the body to not regulate temperature properly and rapidly overheat as well. Drink 1-3 gallons of water daily depending on DNP dose.
Water retention- this is very closely associated with heat. When the vasodilation occurs due to the rise in body temperature, blood vessels expand, causing an increase in blood volume and subsequent water retention. Also, an increased blood volume leads to decreased pressure, which would lead the body to try to store more sodium and cause even more water retention.
All the water retention will subside within a week after stopping the DNP dosage, but often sooner than that. Popular diuretics are not very effective against DNP induced water retention because these diuretics mainly focus on one aspect of diuresis and that is suppression of the anti diuretic hormone (ADH), but the cause of water retention from DNP is independent of ADH.
While diuretics will get rid of some naturally stored water, it isnt getting rid of enough water that would make a competitor presentable on stage and would put the user in jeopardy of death or serious health complications due to potassium depletion.
Lethargy- This is the biggest problem associated with DNP and is somewhat associated with the insomnia that I will cover later. As you have learned DNP depletes the body of ATP and without ATP you have no energy. It literally feels like youre jogging a marathon all day long without a break.
Of course the extent of the lethargy will depend on the dose, but it is not uncommon for people to be almost bed ridden. Walking to the kitchen to get food will be a chore. Even eating the food can become very laborious. This will subside within 24-36 hours of stopping the doses.
Insomnia- sleeping will be very difficult for some people, not because of the familiar central nervous stimulation experienced with ephedrine and caffeine supplementation, but because it gets so damned hot. Many people including myself find it very difficult to sleep when were sweating in our beds.
The best way to combat this is to sleep with 2 fans from both sides of the bed and the air conditioner cranked up. Obviously if you have a significant other that you sleep with then it would be wise to sleep in separate beds for parts of the cycle.
Shortness of breath/ rapid breathing- this is common when the dose is at the upper limits. The breathing will seem like youre jogging even while youre sitting down and doing nothing. It will seem like you can never catch your breath. Doing anything active will make you even more out of breath and this can become dangerous.
When breathing becomes irregular, you should avoid doing any aerobic or strenuous activities. This means no working out (not like youll have any energy to do so anyway).
Dehydration- a very serious side effect. If hydration levels are not adequate it can predispose the body to severe overheating and possibly death. Water needs to be replenished on the order of 1-3 gallons per day.
Electrolyte depletion- this is caused by excessive water and salt loss through sweating. Drinking water will replace fluids, but not electrolytes. Best way to replenish salts is to drink v8 juice.
This can lead to a host of other problems if not remedied including excessive lethargy, low blood pressure, poor cardiac function, nausea, diarrhea
Nausea- This is a common side effect that afflicts roughly around 30% of the users. There could be several causes to this: dehydration, electrolyte imbalance, low blood pH, and other unknown (by me) mechanisms.
Diarrhea- possibly due to electrolyte imbalance and undissolved DNP that passes onto the large intestine causing osmotic imbalances. If this becomes too problematic the only thing to do is just to decrease the dosage or stop completely.
Headache- largely due to dehydration. In most people, forcing down a liter of fluids will alleviate the headaches.
Dry/ sore throat- I dont know the cause of this one, but it is pretty common among users and seems to manifest itself the most during sleep and may contribute to the insomnia.
Allergies/ dermatitis- this is relatively rare. Ive been in contact with nearly 500 people who have used DNP and I would estimate about 30-40 of them have experienced allergic reactions to DNP. The allergies manifest themselves first as phantom itches (itching without any rashes or redness) around the torso in some people. It will later develop into rashes and or hives around the body and possibly spread to the face, neck, lips, and scalp area in severe cases.
Any over the counter or prescription allergy medication (anti histamine) will cure the allergies. Also if youre allergic to DNP it doesnt mean you cant use it in the future. Allergies to DNP seem to have a tolerance factor. It first gets worse, then better with successive cycles.
So if you are allergic, stop immediately and start again 7-10 days later and repeat until you are no longer allergic to DNP anymore. Allergies are also dose and length dependent.
Yellow vision- This is even more rare than allergies. Ive only known about 15 people who have experienced this out of all the people I have come in contact with who have used DNP in the past.
It seems to be most apparent when you look at a white surface and yellow spots will appear on the white that you see. Im not sure what exactly causes this, but it doesnt seem to harm anything and goes away within 1-2 days of stopping the doses.
PREVENTION/ CONTRAINDICATIONS:
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Never start your first cycle with an optimal dose. Always play it safe and start low.
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Never use DNP if youre going to be in a hot environment for an extended period of time.
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Never take any diuretics while on DNP. This includes excessive alcohol. While mild diuretics like alcohol will make you much more uncomfortable and hotter, a harsh diuretic like lasix will kill you when taken with DNP.
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If oral temperature rises to 103 then discontinue use until temp is completely down to normal.
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Do not attempt to work out very intensely. When its hard to find the energy to go to work, dont push yourself thinking you can get a good workout in. Long cardio sessions can be especially harmful for your health. It would also raise cortisol levels through the roof and will be very catabolic to muscle.
Dont sweat the cardio when on DNP because DNP will make you burn fat. Stay away from the treadmill!
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If allergies arise take some allergy medication and if that isnt strong enough then stop the doses for at least 10 days before restarting.
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Watch your electrolytes. Carry a bottle of v8 juice with you. One 8-ounce serving of v8 has 900mg of potassium compared to 35mg of potassium in 8 ounces of Gatorade.
Aim for 3000-5000mg of potassium (not all from v8) per day. Fresh meats and vegetables also have a lot of potassium in them. Sodium is very important too, but is usually not hard to get in the diet. Magnesium can be obtained from supplementation.
- Hydration. I cant emphasize this enough. Not only will proper hydration levels make you feel better and prevent overheating, but it will also make the cycle more effective at burning fat.
SUPPLEMENTATION:
Antioxidantsone of the most effective will be the fat soluble vitamin E. I recommend 800 to 1000 iu of vitamin E per day of the cycle to combat the host of free radical damage caused by increased fat oxidation.
Glycerolthis can be important to help maintain muscle hydration and prevent catabolism. It comes in liquid and can be bought over the counter. Take 3-4 tablespoons per day.
Potassium citrateif blood acidity becomes a problem then potassium citrate can help buffer the acid. About 2-3 grams will be very effective, but 1 gram will do the trick as well.
Ephedrine–this can cause increased mobilization of fatty acid from the adipose cells to get them into the blood where they will be used for energy and burned. In short, ephedrine puts the fat in a place where DNP can burn it.
CONCLUSION
DNP is the most effective fat burner and perhaps the most complicated drug in the bodybuilding community and should not be taken to lightly by average dieters striving to lose a couple of pounds.
The side effects are serious and numerous, but if used correctly, none of the side effects are permanent. Despite these numerous side effects people still use it because it works when nothing else will. I hope this article sufficiently educated you on DNP.
If you choose to use it please do so with caution and use this and other literature as a guide to help you on your way to a new physique.
Good Luck
The Guru
"
HOW TO USE DNP:
"
DNP for Dummies
HOW TO NOT **** UP DNP:
Since some guys have been playing around and disrespecting DNP and then griping to the forums about the painful results, we need to make this VERY specific and VERY correct so that people won’t keep jumping for DNP out of curiosity, or without the willpower they need to operate this responsibly. So here are my experienced guidelines to using it the RIGHT way.
FIRST GUIDLINE: Dosing. Use ONLY 200mg a day for the first four days. I don’t care that you don’t “feel” anything yet and you wanna bump it up. DNP accumulates in the body, and not “feeling” something means NOTHING. It’s there, and it’s working (the effect on metabolism begins within two hours of the first dose!). Four days will let you test your tolerance: do you have an allergy? Does it give you a rash? etc.
Only after those four days do you bump it up, by 200mg a day. The average dose is 400-600 per day, and more than that gets a little severe. A full gram is the highest dose I’ve heard anyone use. I’ve used that much, and it’s hell. I like to stay around 600 a day, which is HOT but safe and effective.
Take caps even hours apart through the day, ending about 4-5 PM. SECOND GUIDLINE…How to eat on DNP. This is purely personal experience, because some guys like to carb-deplete before using DNP (then eat carbs as usual while on), and other guys like a low-carb approach throughout.
Both are fine. Using DNP is the only time that fructose is a
desireable cutting carb, because it keeps the liver replentished. That reduces lethargy and spares muscle.
Be aware that eating high-carb foods WILL increase the heat sensation within an hour, and last about 2 hours.
That means don’t eat carbs before bed unless you want those night sweats to be even WORSE. Personally, I ate whatever the hell I wanted! Chinese, fajitas…Yes, I burned hot, but I still lost 1.5 pounds every 2 days. Keep protein HIGH for muscles’ sake, and try it yourself.
Foods I suggest including:
Blueberry yogurt. Blueberries are excellent antioxidants, and yogurt cultures help with digestive function, gas, and stool consistency (disgustingly soft stools are common during DNP).
Oregano-based foods. Oregano is perhaps one of the most potent antioxidants around, and one spoonful counts as a vegetable serving. Pineapple - I’ve found that pineapple helps alleviate those “DNP Blues”.
The fructose helps, and pineapple enzymes aid in protein digestion. V8 - one 12-ounce can supplies six servings of veggies, concentrated as an excellent source of antioxidants, lycopene, and recovery of electrolytes.
Oatmeal - high-fiber foods are necessary. You’ll find out why around, oh, day 5 or so. Trust me.
THIRD GUIDELINE…Supplements and DNP. I suggest:
ECA - DNP is not a stimulant. To keep energy high and aid in fat loss, use an ECA. Some advisors suggest that regular ephedrine is preferable to norephedrine because of the more direct “hit” of energy.
Prohormones - perfectly fine on DNP. I used 1-AD just to help keep strength and muscle up, and it worked fine. No problems here. You won’t GROW muscle on DNP, but it’ll help with strength and protection.
Obvious stuff - multivitamin, ZMA, etc.
Biotest PowerDrive - No, I’m not pimping Biotest. But PowerDrive is an excellent pre-workout mixture that actually works. Plus it’s low-carb (only 15 calories total), so it won’t cause carb-heat in the middle of your workout.
Antioxidants - I’m giving my own personal list, and why I use them:
Alpha Lipoic Acid - aids in fat management and blood sugar, and an excellent antioxidant.
Grape seed extract
Syntrax Radox
Green Tea
Inositol - mood enhancement, antioxidant, and muscle support. 1 gram/3x day
Ellagic acid - protects cell DNA/RNA from damage by free radicals, and may even attack cancerous cells. 400mg/twice a day
Fruit antioxidants - ****************'s powder of high-potency natural fruit anti’s. 1 gram, 2-3x day.
Trimethylglyceine - antioxidant, helps move fat and blood lipids into the liver and out of the body. 500mg, 2-4x day.
Vitamins E and C
Supplements NOT to use:
Any medications that suppress energy. No allergy meds, antidepressants, muscle relaxers, or beta blockers. DNP will have you low as it is; don’t worsen your body’s energy by taking something that suppresses you further.
DRUGS - Sheesh, you’d think I wouldn’t have to mention this, but two idiots in particular (on Elite Fitness) recently affirmed that some people still just don’t get it.
NO alcohol (not even “moderate”), NO ecstasy, NO GHB, etc. If you don’t have the willpower to forego these habits,
DNP is not for you.
Syntrax Swole - a personal discovery. I tried Swole while on DNP…once. Two hours of hell, feeling inside-out.
FOURTH GUIDELINE…working out on DNP. Keep lifting short, 30-40 minutes. DNP works very well, causing your body to use 150% of the calories per action you’d normally use. That means DON’T try to repeat your usual workouts. Drop to moderate weights, 8-12 reps, not to failure, and with plenty of walking rest between sets.
You are NOT going to grow muscle on DNP, so don’t use your usual heavy routine. Since DNP can cause light-headedness and heat dizzyness, you have my permission to skip squats in favor of leg presses this time.
Cardio is a controversial one. My advice - do NOT do cardio on high doses of DNP (600mg or more). It’s dangerous and counterproductive. Below that amount, some cardio is fine, but keep it to 20 minutes and not at full-gallop. Remember, DNP will drain water from your quickly, causing you to leech out minerals, vitamins, and salts. Don’t overdo it.
During exercise, consume at least 1 liter of water per 30 minutes of work, whether you’re thirsty or not.
DNP is evil in the way it blunts thirst, while at the same time doing the cruel trick of bloating your body with water WHILE dehydrating you from water in your organs. MAKE yourself drink. Always folllow DNP exercise with antioxidants, carbs, and this is a good time to use your multivitamin.
Don’t feel embarrassed about poor workouts. Just this morning I did a workout with a whopping nine sets (wimp!) before calling it quits. Listen to your body, and let it tell you when enough’s enough; don’t gauge workouts by what you usually can do otherwise.
Purely for information’s sake. Posting any of this information is not an admission that I have ever used DNP; I refer to myself in the first person as a hypothetical narrator in an academic discourse…
DNP is Ames negative, and does not promote tumors. See for yourself at http://toxnet.nlm.nih.gov/
Technical Air Pollution Resources | US EPA reports on health risks. While there have not been human studies, animal studies found no cancers caused by DNP administration. It is considered a toxin because it causes nausea, sweating, and weight loss.
DNP and Bodybuilding, Dinitrophenol Information reports on health risks from external exposure. In other words, dont get it in your eyes, or on your skin if youre allergic. Pretty elementary stuff.
http://www.ebec2000.com/abstracts/056.htm This animal study documents a 64% increase in metabolism. “These findings confirm that DNP effectively increases metabolic rate…” Duh.
Antibodies | Thermo Fisher Scientific - US A PDF file about an antidote to DNP.
http://www.boehringer-ingelheim.es/w...lesa/cap13.htm finds that DNP did not activate liver enzymes (MAT) associated with liver damage
“Comparative study of toxicity of 4-nitrophenol and 2,4-dinitrophenol in newborn and young rats.” Koizumi M, Yamamoto Y, Ito Y, Takano M, Enami T, Kamata E, Hasegawa R. Division of Risk Assessment, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
This study found that DNP can induce death in overdosed amounts, but that up to that point no toxicity was evident, nor were there any abnormalities in physical development.
“Phenol toxicity and conjugation in human colonic epithelial cells.” Pedersen G, Brynskov J, Saermark T. Dept of Medical Gastroenterology, Herlev University Hospital, Copenhagen, Denmark… This study found that DNP has a toxic effect on cells of the colon, with “toxic” defined in two ways: first, it interfered with metabolism (this we knowits the intended effect of DNP users!) and second, it interfered with bowel inflammation (not a health risk.
This is caused by osmotic effect, with the worst result being softened stools and gas).
“Mechanisms of bacterial resistance to macrolide antibiotics.” Nakajima Y. Division of Microbiology, Hokkaido College of Pharmacy, 7-1 Katsuraoka-cho, Otaru, Hokkaido 047-0264, Japan. This study found that antibiotic-resistant bacteria could be thwarted with DNP.
“the extent of the accumulated drug in a resistant cell increases as much as that in a susceptible cell in the presence of an uncoupling agent such as2,4-dinitrophenol (DNP).”
“Absence of Crabtree effect in human melanoma cells adapted to growth at low pH: reversal by respiratory inhibitors.” Burd R, Wachsberger PR, Biaglow JE, Wahl ML, Lee I, Leeper DB. Departments of Radiation Oncology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Check this outDNP actually helps make melanoma tumors easier to attack by increasing ratio of oxygen consumption to lactic acid production, while glycolysis remains the same. “Therefore, tumor acute acidification and oxygenation can be achieved by exposure”
“New insights in the cellular processing of platinum antitumor compounds, using fluorophore-labeled platinum complexes and digital fluorescence microscopy.”
Molenaar C, Teuben JM, Heetebrij RJ, Tanke HJ, Reedijk J. Department of Molecular Cell Biology, Leiden University Medical Centre, The Netherlands. DNP is used as a control in tests of antitumor cells because it does NOT bind to cell DNA, nor promote tumors, yet its staining abilities enable tracking of the uptake of antitumor drugs.
Specific inhibition of breast cancer cells by antisense poly-DNP-oligoribonucleotides and targeted apoptosis." Ru K, Taub ML, Wang JH. Department of Biochemistry, State University of New York, Buffalo 14260-3000, USA Are you ready for this? DNP actually INHIBITS (!!!) breast cancers!
Yes, not only does it NOT promote cancers, its being recognized as a cancer-fighter/blocker.
"Two membrane-permeable and RNase-resistant antisense poly-2’-O-(2,4-dinitrophenyl)-oligoribonucleotides (poly-DNP-RNAs) have been synthesized as inhibitors of human breast cancerfluorescence assay indicates that the targeted antisense inhibition by poly-DNP-RNAs leads to apoptosis of SK-Br-3 cells but does not affect nontumorigenic MCF-10A cells.
The control poly-DNP-RNAs with random or sense nucleotide sequence are completely inactive." Plain English?
DNP is being synthesized as an anti-cancer compound, because tests show that it blocks mutagens but does NOT affect non-mutagenic (healthy) cells, and has no RNA effects on them.
“Heat shock protein induction by certain chemical stressors is correlated with their cytotoxicity, lipophilicity and protein-denaturing capacity.” Neuhaus-Steinmetz U, Rensing L. Institute of Cell Biology, Biochemistry and Biotechnology, NW II University of Bremen, Germany.
The thermic effect of DNP induces protein synthesis (heat shock protein, or HSP, synthesis). In fact, its quite GOOD at it: “ASA, DNP and CCCP induced HSP at lower concentrations than substances with a similar lipophilicity”
“Comparative effects of the metabolic inhibitors 2,4-dinitrophenol and iodoacetate on mouse neuroblastoma cells in vitro.” Andres MI, Repetto G, Sanz P, Repetto M.
National Institute of Toxicology, Seville, Spain. In this study, DNPs observed effect was an increase in metabolism (duh!), while the other toxins compared to it had harmful in vitro effects but no increase in metabolism.
“Inhibition of uncoupled respiration in tumor cells. A possible role of mitochondrial Ca2+ efflux.” Gabai VL.Medical Radiology Research Center, Russian Academy of Medical Sciences, Obninsk. DNP not only does not cause tumors, but it inhibited their respiration by 20-25% compared to controls.
“Amsacrine-induced lesions in DNA and their modulation by novobiocin and 2,4-dinitrophenol.” Shibuya ML, Buddenbaum WE, Don AL, Utsumi H, Suciu D, Kosaka T, Elkind MM. Department of Radiology and Radiation Biology, Colorado State University, Fort Collins 80523.
In this study, researchers found that DNP abrogatesor disruptscytotoxicity in hamsters (using cancerous cells). They expected to find that DNP would interfere with anticancer treatments, but instead found that DNP increased their effects.
They state, though, that they cannot claim a proven effect of DNP on anticancer treatments yet, although they do agree that treatment with DNP actually enhanced the effects of the DNA regenerative therapy of anticancer chemotherapy.
“Induction of endonucleolytic DNA cleavage in human acute myelogenous leukemia cells by etoposide, camptothecin, and other cytotoxic anticancer drugs: a cautionary note.” Kaufmann SH. Oncology Center, Johns Hopkins Hospital, Baltimore, Maryland 21205.
The authors warn that certain anti-leukemia drugs resulted in “extensive DNA degradation.” BUT(good ol DNP to the rescue!), “Preincubation with dinitrophenol abolished the effect”
“[Dependence of the nature of the action of metabolic inhibitors on ribosomal RNA synthesis in Ehrlich ascites carcinoma cells on cell integrity]” [Article in Russian] Akhlynina TV, Buzhurina IM, Panov MA, Rozovskaia IA, Chernaia NG. DNP actually inhibits the synthesis of RNA in carcinoma cells.
In other words, it helps cancerous cells commit suicide. “Ribosomal RNA (rRNA) synthesis in the intact Ehrlich ascite carcinoma cells is selectively inhibited by papaverin (ED50 = 0.01 mM), 2,4-dinitrophenol (DPN; ED50 = 5 microM), and actinomycin D (ED50 = 0.1 microgram/ml).”
“Autocatabolism of surface macromolecules shed by human melanoma cells.”
Bystryn JC, Perlstein J. Cancer Res 1982 Jun;42(6):2232-7. This study finds that DNP helps
melanoma cells die (autocatabolize) while other cells are unaffected.
Yahoo | Mail, Weather, Search, Politics, News, Finance, Sports & Videos - tons of research, including medical studies. Excerpts:
DNP does not cause liver damage: “Their analyses demonstrate, beyond a doubt, that the liver does not suffer any damage in the course of dinitro treatment.”
(Biological Study of Dinitro Drugs in Humans By Dr. Jacques Bell. Bell, Jacques. 1939. Etude biologique des produits dinitres chez l’homme. Medecine. 19:749-54. Translation 1996 Robert Ames)
Also: "Experimental studies on animals do not show toxic effects of dinitrophenol on the kidney. Anatomical-pathological examinations of animals, even those which died from a massive dose of dinitrophenol, do not reveal any important anatomical changes, except a small degree of cytolysis.
Clinical documents are not abundant, but, on the whole, do not seem to demonstrate that dinitrophenol is toxic for the kidneys."
“Dinitrophenol has almost no action on the blood cholesterol. (Grant and Schube).”
“it doesn’t seem that dinitrophenol at usual clinical doses is likely to harm the kidneys.”
“Dinitrophenol is remarkable for its absence of effect on the cardio-vascular system…dinitrophenol is absolutely devoid of toxicity for the heart.”
“Dinitrophenol does not attack cell tissue albumin and does not determine the fat loss to the expense of the muscles, contrary to thyroxine.”
“dinitrophenol offers this precious advantage that the cessation of its use at the slightest appearance of signs indicating an imminence of intoxication results immediately in the arrest of those symptoms.” (Professor Pouchet)."
Interestingly, one medical theory on a health ADVANTAGE of DNP is that the slight increase in thermogenic temperature simulates the fever a body induces during a viral attack.
The body increases itsheat to protect organs but kill viruses, and some theorize that DNP can do the same thing, thus killing viruses in the body. In this mechanism, DNP may have an immune-enhancing effect.
" [original post by: Smokinghawk]