Mitochondrial Decay in Aging: Reversal through Supplementation of Acetyl-l-Carnitine and N-tert-Butyl--phenyl-nitronea
TORY M. HAGENc, CAROL M. WEHRd and BRUCE N. AMESb
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
aThis work was supported by a Grant from the Sandoz Gerontological Foundation (T.M.Hagen); by NCI Outstanding Investigator Grant CA, 39910; and NIEHS Center Grant, ES01896.
bTo whom correspondence should be addressed. Tel: 510/642-5165; fax: 510/643-7935; e-mail; firstname.lastname@example.org
cCurrent address: linus Pauling Institute at Oregon State University, 571 Weniger Hall, Corvallis, OR 97331. Tel:541/737-5083; fax: 541/737-5077; e-mial; email@example.com
We show that mitochondrial function in the majority of hepatocytes isolated from old rats (24 mo) is significantly impaired.
Mitochondrial membrane potential, cardiolipin levels, respiratory control ratio, and overall cellular O2 consumption decline, and the level of oxidants increases.
To examine whether dietary supplementation of micronutrients that may have become essential with age could reverse the decline in mitochondrial function, we supplemented the diet of old rats with 1% (w/v) acetyl-l-carnitine (ALCAR) in drinking water.
ALCAR supplementation (1 month) resulted in significant increases in cellular respiration, mitochondrial membrane potential, and cardiolipin values. However, supplementation also increased the rate of oxidant production, indicating that the efficiency of mitochondrial electron transport had not improved.
To counteract the potential increase in oxidative stress, animals were administered N-tert-butyl-a-phenyl-nitrone (30 mg/kg) (PBN) with or without ALCAR. Results showed that PBN significantly lowered oxidant production as measured by 2,7'-dichlorofluorescin diacetate (DCFH), even when ALCAR was coadministered to the animals.
Thus, dietary supplementation with ALCAR, particularly in combination with PBN, improves mitochondrial function without a significant increase in oxidative stress.
Note: for us it points to upping the anti-oxidants if we're going to crank our fat burning potential via mitochondrial revitalization . . .